Biochemical and Molecular Characterization of Obesumbacterium proteus, a Common Contaminant of Brewing Yeasts.

The taxonomic position of Obesumbacterium proteus, a common brewery contaminant.

MALDI-TOF MS typing enables the classification of brewing yeasts of the genus Saccharomyces to major beer styles

Brewing yeasts of the genus Saccharomyces are either available from yeast distributor centers or from breweries employing their own "in-house strains". During the last years, the classification and characterization of yeasts of the genus Saccharomyces was achieved by using biochemical and DNA-based methods. The current lack of fast, cost-effective and simple methods to classify brewing yeasts t...

Gene cloning, expression and characterization of novel phytase from Obesumbacterium proteus.

The gene phyA encoding phytase was isolated from Obesumbacterium proteus genomic library and sequenced. The cleavage site of the PhyA signal peptide was predicted and experimentally proved. The PhyA protein shows maximum identity of 53% and 47% to phosphoanhydride phosphorylase from Yersinia pestis and phytase AppA from Escherichia coli, respectively. Based on protein sequence similarity of Phy...

Genetic Diversity of Proteus Mirabilis Isolated from Urine Specimens by Box PCR

Introduction: Proteus mirabilis is one of the common causes of UTI.Multiple molecular methods are used to genotype bacteria.The polymerase chain reaction method is based on repeated sequences using BOXA1R primers for differentiation of bacteria. This study aimed to evaluate the genetic ‎diversity of protease mirabilis isolated from urine specimens.   Materials & Methods: In this descriptive cr...

Recombinant expression, purification, and biochemical characterization of chondroitinase ABC II from Proteus vulgaris.

Chondroitin lyases (or chondroitinases) are a family of enzymes that depolymerize chondroitin sulfate (CS) and dermatan sulfate (DS) galactosaminoglycans, which have gained prominence as important players in central nervous system biology. Two distinct chondroitinase ABC enzymes, cABCI and cABCII, were identified in Proteus vulgaris. Recently, cABCI was cloned, recombinantly expressed, and exte...